CHOK1-GenS&CHOK1-ADCC+
Cell Line Development System Commercial License
ProBio has 12 years of experience in mammalian cell line development, and is one of the earliest companies getting the CHO-K1 – CHO commercial license for commercial use and sublicense agreement from ECACC.
ProBio has two cell line development systems: wild-type cell line CHOK1-GenS and ADCC-enhanced cell line CHOK1-ADCC +. CHOK1-GenS cell line development system without chemical . Developed ADCC enhanced host cell line (CHOK1-ADCC+), which can produce afucosylated monoclonal antibodies with enhanced ADCC activity. They are in together with the high-expression proprietary pGenHT 1.0-UP vector, the average titer of mAb is 5.7 g/L, the maximum titer of mAb is up to 15 g/L.
CHOK1 system exclusive advantages
IP Clear
• ECACC license agreement• Proprietary pGenHT 1.0-UP vector
Royalty Free
• One-time payment without royalty
2 CHO-K1 Host Cell License to Choose
• Wide type CHOK1-GenS• ADCC enhanced CHOK1-ADCC+
Combo license at discounted price
• Purchase in combination with wild type CHOK1-GenS cell line, and get a discounted price
Suitable for different type of proteins
• mAb/Protein
• BsAb,etc
Fulfill the regulatory requirements
• Fulfill the regulatory requirements of FDA,EMA and NMPA
Case Study of CHOK1-GenS cell line system
High productivity with CHOK1-GenS system
- Suitable for different type of molecules
Excellent stability with CHOK1-GenS system
- The titer at the end of stability study during fed-batch is recorded as T2.
- The titer at the beginning of stability study during fed-batch is recorded as T1.
- Titer variation=(T2-T1)/T1*100%
- Titer variation should be within 30%.
High productivity with CHOK1-GenS system
Titer prior to cell culture up to 15 g/L
Excellent Stability in Scale-Up from 3L to 200L
Case Study of CHOK1-ADCC+ cell line system
CD16a 158V/F affinity (BLI)
The affinity to CD16a (158V and 158F) was increased by 3-4 times.
PBMC-based ADCC
- Cytotoxicity is increased by about 10%.
- Effect concentration (EC50) was reduced by 6 times.
- ADCC effect was significantly enhanced.
CHOK1 delivery package
Media Contact
Company Name: ProBio CDMO
Email: Send Email
Phone: 1-877-436-7274 (Toll-Free)
Country: United States
Website: https://www.probiocdmo.com